9V4J
Prenyltransferase Ord1 Wild type-FSPP
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE BL-1A |
| Synchrotron site | Photon Factory |
| Beamline | BL-1A |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2021-10-31 |
| Detector | DECTRIS EIGER X 4M |
| Wavelength(s) | 1.04500 |
| Spacegroup name | P 1 |
| Unit cell lengths | 59.100, 72.560, 84.220 |
| Unit cell angles | 94.04, 69.48, 90.04 |
Refinement procedure
| Resolution | 44.530 - 2.000 |
| R-factor | 0.2412 |
| Rwork | 0.238 |
| R-free | 0.27220 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 9v4g |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.051 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | MOLREP |
| Refinement software | PHENIX (1.19.2.4158) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.120 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.052 | 0.706 |
| Number of reflections | 86258 | 13824 |
| <I/σ(I)> | 12.71 | 1.75 |
| Completeness [%] | 97.6 | 96.6 |
| Redundancy | 3.6 | 3.7 |
| CC(1/2) | 0.999 | 0.746 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5.5 | 291 | 0.1 M Bis-Tris, pH 5.5, 0.2 M Ammonium sulfate, 25% PEG3350 |
