9V4I
Prenyltransferase Ord1 D219A
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE BL-17A |
| Synchrotron site | Photon Factory |
| Beamline | BL-17A |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2023-06-03 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.980000 |
| Spacegroup name | P 1 |
| Unit cell lengths | 58.879, 72.425, 83.774 |
| Unit cell angles | 93.45, 110.50, 90.08 |
Refinement procedure
| Resolution | 44.350 - 2.800 |
| R-factor | 0.2307 |
| Rwork | 0.224 |
| R-free | 0.25390 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 9v4g |
| RMSD bond length | 0.004 |
| RMSD bond angle | 0.724 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | MOLREP |
| Refinement software | PHENIX (1.19.2.4158) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.970 |
| High resolution limit [Å] | 2.800 | 2.800 |
| Rmerge | 0.118 | 0.712 |
| Number of reflections | 31241 | 4990 |
| <I/σ(I)> | 11.83 | 3.09 |
| Completeness [%] | 97.8 | 97.2 |
| Redundancy | 3.6 | 3.7 |
| CC(1/2) | 0.996 | 0.771 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5.5 | 291 | 0.1 M Bis-Tris, pH 5.5, 0.2 M Ammonium sulfate, 25% PEG 3350 |
