9V2T
Crystal Structure of avermectin oxidase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL10U2 |
| Synchrotron site | SSRF |
| Beamline | BL10U2 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2025-03-03 |
| Detector | DECTRIS EIGER R 1M |
| Wavelength(s) | 0.979183 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 176.614, 47.540, 107.834 |
| Unit cell angles | 90.00, 99.82, 90.00 |
Refinement procedure
| Resolution | 42.250 - 3.150 |
| Rwork | 0.253 |
| R-free | 0.30820 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Data scaling software | pointless (1.12.16) |
| Refinement software | PHENIX (1.15.1_3469) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 42.250 | 3.263 |
| High resolution limit [Å] | 3.150 | 3.150 |
| Rmerge | 0.753 | |
| Rmeas | 0.882 | |
| Rpim | 0.453 | |
| Number of reflections | 15514 | 1524 |
| <I/σ(I)> | 5.16 | |
| Completeness [%] | 98.8 | 99.67 |
| Redundancy | 3.5 | 3.7 |
| CC(1/2) | 0.967 | 0.496 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 291.15 | PEG 8000,Sodium cacodylate trihydrate, Magnesium acetate tetrahydrate |
