9V17
Crystal structure of E. coli glycogen phosphorylase N185A/R267E mutant
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL44XU |
| Synchrotron site | SPring-8 |
| Beamline | BL44XU |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2025-04-23 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.9 |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 187.297, 187.297, 449.824 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 49.600 - 3.700 |
| R-factor | 0.1975 |
| Rwork | 0.196 |
| R-free | 0.22940 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.642 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | MOLREP |
| Refinement software | PHENIX (1.19.2_4158) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 49.600 | 3.770 |
| High resolution limit [Å] | 3.700 | 3.700 |
| Rmerge | 0.248 | 2.950 |
| Rmeas | 0.262 | 3.130 |
| Rpim | 0.082 | 0.995 |
| Number of reflections | 84810 | 4444 |
| <I/σ(I)> | 9 | 1.1 |
| Completeness [%] | 98.9 | 99.3 |
| Redundancy | 9.2 | 9 |
| CC(1/2) | 0.997 | 0.782 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | 2% v/v 1,4-dioxane, 0.1M Tris pH 8.0, 15% w/v polyethylene glycol 3350 |
