9V16
Crystal structure of E. coli glycogen phosphorylase N185A/R267E mutant in complex with AMP
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL44XU |
| Synchrotron site | SPring-8 |
| Beamline | BL44XU |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2025-04-23 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.9 |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 185.604, 185.604, 451.242 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 49.450 - 3.450 |
| R-factor | 0.1942 |
| Rwork | 0.192 |
| R-free | 0.23510 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.408 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | MOLREP |
| Refinement software | PHENIX ((1.19.2_4158: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 49.500 | 3.510 |
| High resolution limit [Å] | 3.450 | 3.450 |
| Rmerge | 0.164 | 2.000 |
| Rmeas | 0.173 | 2.110 |
| Rpim | 0.054 | 0.652 |
| Number of reflections | 98722 | 4913 |
| <I/σ(I)> | 12 | 1.5 |
| Completeness [%] | 95.3 | 96.7 |
| Redundancy | 9.6 | 10 |
| CC(1/2) | 0.998 | 0.810 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | 2% v/v 1,4-dioxane, 0.1M Tris pH 8.0, 15% w/v polyethylene glycol 3350 (The crystal was soaked in a solution supplemented with 40 mM AMP before data collection) |
