9V11
The Fab fragment of Osemitamab (TST001), a therapeutic antibody targeting claudin 18.2
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL19U1 |
| Synchrotron site | SSRF |
| Beamline | BL19U1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2024-03-23 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.98655 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 36.512, 106.144, 112.148 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 47.972 - 1.728 |
| R-factor | 0.2008 |
| Rwork | 0.200 |
| R-free | 0.22860 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.851 |
| Data reduction software | XDS (BUILT 20230630) |
| Data scaling software | Aimless (0.7.4) |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.13_2998: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 47.972 | 1.758 |
| High resolution limit [Å] | 1.728 | 1.728 |
| Rmerge | 0.109 | 1.423 |
| Rmeas | 0.120 | 1.480 |
| Rpim | 0.031 | 0.402 |
| Number of reflections | 46681 | 2286 |
| <I/σ(I)> | 12.1 | |
| Completeness [%] | 100.0 | |
| Redundancy | 13.1 | 13.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 293.15 | 0.5 M ammonium sulfate, 0.1 M HEPES pH 7.5, 30% v/v (+/-)-2-Methyl-2,4-pentanediol |
