9V0W
UDP-Glc:tetrahydrobiopterin glucosyltransferase from Pseudanabaena sp. Chao 1811
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2024-07-28 |
| Detector | DECTRIS PILATUS 200K |
| Wavelength(s) | 1.54056 |
| Spacegroup name | P 61 2 2 |
| Unit cell lengths | 162.213, 162.213, 181.952 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 13.250 - 2.800 |
| R-factor | 0.2412 |
| Rwork | 0.240 |
| R-free | 0.27230 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.857 |
| Data reduction software | CrysalisPro (1.171.39.35c) |
| Data scaling software | Aimless (0.8.2) |
| Phasing software | MOLREP (11.9.02) |
| Refinement software | PHENIX ((1.20.1_4487)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 13.250 | 2.900 |
| High resolution limit [Å] | 2.800 | 2.800 |
| Rpim | 0.044 | 0.576 |
| Number of reflections | 34964 | 4609 |
| <I/σ(I)> | 18.3 | 2 |
| Completeness [%] | 99.0 | |
| Redundancy | 15 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8 | 285 | ammonium sulfate 1M, Tris 0.1M pH 8.0 |
