9UZG

Crystal structure of Kasokero virus cap- snatching endonuclease in complex with 2,4-dioxo-4-phenylbutanoic acid (DPBA)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL10U2
Synchrotron site	SSRF
Beamline	BL10U2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2024-10-27
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.97918
Spacegroup name	P 1 21 1
Unit cell lengths	37.224, 80.027, 115.771
Unit cell angles	90.00, 93.19, 90.00

Refinement procedure

Resolution	28.900 - 2.150
R-factor	0.1837
Rwork	0.181
R-free	0.23260
Structure solution method	SAD
RMSD bond length	0.007
RMSD bond angle	0.969
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	PHENIX ((1.20.1_4487: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	29.780	2.220
High resolution limit [Å]	2.150	2.150
Rmerge	0.105	0.353
Rmeas	0.148	0.495
Rpim	0.104	0.346
Number of reflections	33374	3178
<I/σ(I)>	4.8
Completeness [%]	90.2	99
Redundancy	3	3
CC(1/2)	0.984	0.803

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	EVAPORATION		289	25% vol/vol Jeffamine ED2003, 0.2 M NaCl, and 0.1 M MES-NaOH pH 6.0