9UZF

Crystal structure of Kasokero virus cap- snatching endonuclease (D719A mutant)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL02U1
Synchrotron site	SSRF
Beamline	BL02U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2025-01-03
Detector	DECTRIS EIGER2 S 9M
Wavelength(s)	0.97914
Spacegroup name	P 1 21 1
Unit cell lengths	37.120, 81.150, 116.800
Unit cell angles	90.00, 97.65, 90.00

Refinement procedure

Resolution	29.330 - 2.470
R-factor	0.2029
Rwork	0.200
R-free	0.25700
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.009
RMSD bond angle	1.158
Data reduction software	xia2
Data scaling software	xia2
Phasing software	PHASER
Refinement software	PHENIX ((1.20.1_4487: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	29.330	2.570
High resolution limit [Å]	2.470	2.470
Rmerge	0.105	0.658
Rmeas	0.105	0.873
Rpim	0.090	0.569
Number of reflections	24663	2738
<I/σ(I)>	7.5
Completeness [%]	99.6	99.9
Redundancy	3.9
CC(1/2)	0.988	0.988

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	EVAPORATION		298	25% vol/vol Jeffamine ED2003, 0.2 M NaCl, and 0.1 M MES-NaOH pH 6.0