9UZE

Crystal structure of Kasokero virus cap- snatching endonuclease (E682A mutant)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL02U1
Synchrotron site	SSRF
Beamline	BL02U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2025-01-03
Detector	DECTRIS EIGER2 S 9M
Wavelength(s)	0.97914
Spacegroup name	P 1 21 1
Unit cell lengths	37.176, 81.108, 115.871
Unit cell angles	90.00, 93.38, 90.00

Refinement procedure

Resolution	29.850 - 2.410
R-factor	0.181
Rwork	0.178
R-free	0.23340
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.008
RMSD bond angle	1.011
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	PHENIX ((1.20.1_4487: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	29.850	2.500
High resolution limit [Å]	2.410	2.410
Rmerge	0.071	0.399
Rmeas	0.100	0.561
Rpim	0.070	0.394
Number of reflections	24612	2170
<I/σ(I)>	10.8	2.3
Completeness [%]	92.4	78.7
Redundancy	3.1
CC(1/2)	0.994	0.675

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	EVAPORATION		289	25% vol/vol Jeffamine ED2003, 0.2 M NaCl, and 0.1 M MES-NaOH pH 6.0