9UZD

Crystal structure of Kasokero virus cap- snatching endonuclease (E668A mutant)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL02U1
Synchrotron site	SSRF
Beamline	BL02U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2025-01-03
Detector	DECTRIS EIGER2 S 9M
Wavelength(s)	0.97914
Spacegroup name	P 1 21 1
Unit cell lengths	37.189, 80.332, 116.262
Unit cell angles	90.00, 97.74, 90.00

Refinement procedure

Resolution	29.300 - 2.400
R-factor	0.1932
Rwork	0.191
R-free	0.24750
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.008
RMSD bond angle	1.136
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	PHENIX ((1.20.1_4487: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	29.300	2.490
High resolution limit [Å]	2.400	2.400
Rmerge	0.087	0.516
Rmeas	0.111	0.672
Rpim	0.068	0.424
Number of reflections	25265	2229
<I/σ(I)>	9.6
Completeness [%]	94.9	79.1
Redundancy	4.2	3
CC(1/2)	0.991	0.652

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	EVAPORATION		298	25% vol/vol Jeffamine ED2003, 0.2 M NaCl, and 0.1 M MES-NaOH pH 6.0