9UZC
Crystal structure of Kasokero virus cap- snatching endonuclease in complex with manganese ions
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL10U2 |
| Synchrotron site | SSRF |
| Beamline | BL10U2 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2024-09-27 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.97918 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 37.202, 80.659, 114.990 |
| Unit cell angles | 90.00, 93.52, 90.00 |
Refinement procedure
| Resolution | 28.690 - 1.980 |
| R-factor | 0.2311 |
| Rwork | 0.230 |
| R-free | 0.25670 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.040 |
| Data reduction software | xia2 |
| Data scaling software | xia2 |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.20.1_4487: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 28.690 | 2.020 |
| High resolution limit [Å] | 1.980 | 1.990 |
| Rmerge | 0.118 | 0.327 |
| Rmeas | 0.167 | 0.462 |
| Rpim | 0.118 | 0.327 |
| Number of reflections | 89384 | 4174 |
| <I/σ(I)> | 5 | 2.6 |
| Completeness [%] | 97.3 | 89 |
| Redundancy | 2.8 | 2.8 |
| CC(1/2) | 0.973 | 0.874 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | EVAPORATION | 289 | 25% vol/vol Jeffamine ED2003, 0.2 M NaCl, and 0.1 M MES-NaOH pH 6.0 |
