9UZB
Crystal structure of Kasokero virus cap-snatching endonuclease in apo form
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL10U2 |
| Synchrotron site | SSRF |
| Beamline | BL10U2 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2024-09-27 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.97918 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 37.162, 80.906, 115.184 |
| Unit cell angles | 90.00, 93.47, 90.00 |
Refinement procedure
| Resolution | 27.500 - 1.900 |
| R-factor | 0.1842 |
| Rwork | 0.182 |
| R-free | 0.22070 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.080 |
| Data reduction software | xia2 |
| Data scaling software | xia2 |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.20.1_4487: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 28.410 | 1.940 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.141 | 0.451 |
| Rmeas | 0.192 | 0.613 |
| Rpim | 0.128 | 0.412 |
| Number of reflections | 53558 | 3413 |
| <I/σ(I)> | 6.5 | |
| Completeness [%] | 99.5 | 98.3 |
| Redundancy | 3.3 | 3.3 |
| CC(1/2) | 0.972 | 0.629 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | EVAPORATION | 289 | 25% vol/vol Jeffamine ED2003, 0.2 M NaCl, and 0.1 M MES-NaOH pH 6.0 |
