9UZA

Crystal structure of Crimean-Congo hemorrhagic fever virus cap-snatching endonuclease

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL10U2
Synchrotron site	SSRF
Beamline	BL10U2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2024-04-30
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.97918
Spacegroup name	P 21 21 21
Unit cell lengths	98.767, 99.779, 180.419
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	29.110 - 3.050
R-factor	0.2291
Rwork	0.226
R-free	0.29500
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.013
RMSD bond angle	1.619
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	PHENIX ((1.20.1_4487: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	90.210	3.210
High resolution limit [Å]	3.040	3.040
Rmerge	0.149
Rmeas	0.161
Rpim	0.062
Number of reflections	35085	5038
<I/σ(I)>	5.1
Completeness [%]	100.0	100
Redundancy	13.1	13.6
CC(1/2)	0.998	0.434

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	EVAPORATION		289	2% vol/vol TacsimateTM pH 7.0, 0.1 M HEPES pH 7.5, and 20% wt/vol Polyethylene glycol 3,350