9UYR

In situ structure of egg-white lysozyme using a goniometer-compatible chip-based platform

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PHOTON FACTORY BEAMLINE AR-NW12A
Synchrotron site	Photon Factory
Beamline	AR-NW12A
Temperature [K]	298
Detector technology	PIXEL
Collection date	2022-10-22
Detector	DECTRIS PILATUS3 2M
Wavelength(s)	0.75
Spacegroup name	P 43 21 2
Unit cell lengths	79.116, 79.116, 37.925
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	35.380 - 1.590
R-factor	0.1702
Rwork	0.169
R-free	0.19400
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.005
RMSD bond angle	0.733
Phasing software	PHENIX (1.17.1_3660)
Refinement software	PHENIX (1.17.1_3660)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	39.000	1.650
High resolution limit [Å]	1.590	1.590
Number of reflections	16028	1592
<I/σ(I)>	16
Completeness [%]	95.7
Redundancy	6
CC(1/2)	0.990	0.620

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	4.5	293	40mg/mL in 50mM sodium acetate, 0.8M NaCl