9UYC
TxtE mutant-H176FA248F With trp
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL12-2 |
| Synchrotron site | SSRL |
| Beamline | BL12-2 |
| Temperature [K] | 293 |
| Detector technology | PIXEL |
| Collection date | 2024-05-06 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 0.97919 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 75.570, 99.530, 105.680 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 72.450 - 1.530 |
| Rwork | 0.297 |
| R-free | 0.36000 |
| Structure solution method | MAD |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0415) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 72.450 | 1.560 |
| High resolution limit [Å] | 1.530 | 1.530 |
| Number of reflections | 120285 | 5877 |
| <I/σ(I)> | 11.2 | 1.6 |
| Completeness [%] | 99.7 | 99.8 |
| Redundancy | 11.9 | |
| CC(1/2) | 0.998 | 0.683 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | 0.2 M Magnesium chloride hexahydrate, 0.1 M HEPES pH 7.5, 25% w/v Polyethylene glycol 3,350 |
