9UXJ

Structure of WDR5 in complex with Peptide 2_R4A

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL02U1
Synchrotron site	SSRF
Beamline	BL02U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2024-06-23
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.9795
Spacegroup name	C 2 2 21
Unit cell lengths	80.321, 100.923, 81.740
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	31.760 - 1.760
R-factor	0.1863
Rwork	0.184
R-free	0.22450
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.020
RMSD bond angle	1.408
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX ((1.19.2_4158: ???))

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	1.810
High resolution limit [Å]	1.750	3.770	1.750
Rmerge		0.115
Rmeas		0.129
Rpim	0.074	0.057	0.390
Number of reflections	33369	3510	3311
<I/σ(I)>	3
Completeness [%]	100.0	99.8	100
Redundancy	5.8	5.2	5.3
CC(1/2)	0.986	0.987	0.707

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		291	0.2 M imidazole malate (pH 7.0), and 25 % w/v PEG 4000