9UXG
Structure of WDR5 in complex with Peptide 2
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL02U1 |
| Synchrotron site | SSRF |
| Beamline | BL02U1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2024-06-01 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.9792 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 49.122, 46.714, 64.730 |
| Unit cell angles | 90.00, 98.42, 90.00 |
Refinement procedure
| Resolution | 41.760 - 1.700 |
| R-factor | 0.2221 |
| Rwork | 0.221 |
| R-free | 0.24320 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.092 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.19.2_4158: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 46.710 | 1.730 |
| High resolution limit [Å] | 1.700 | 1.700 |
| Rpim | 0.043 | 0.282 |
| Number of reflections | 31378 | 6374 |
| <I/σ(I)> | 12.4 | |
| Completeness [%] | 97.8 | |
| Redundancy | 5.2 | 4.7 |
| CC(1/2) | 0.997 | 0.792 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 291 | 0.2M Sodium iodide, 0.1M Bis-tris propane pH 7.5, 20% w/v PEG 3350 |
