9UWT

Crystal structure of human galectin-10 produced by cell-free protein synthesis in complex with maltose

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL32XU
Synchrotron site	SPring-8
Beamline	BL32XU
Temperature [K]	100
Detector technology	PIXEL
Collection date	2023-01-20
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	1
Spacegroup name	P 65 2 2
Unit cell lengths	49.050, 49.050, 261.970
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	35.640 - 2.310
R-factor	0.2361
Rwork	0.228
R-free	0.31030
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.008
RMSD bond angle	1.101
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	MOLREP
Refinement software	PHENIX (1.19.2_4158)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.450
High resolution limit [Å]	2.310	2.310
Number of reflections	8771	1391
<I/σ(I)>	4.1
Completeness [%]	96.0	97.8
Redundancy	5.2
CC(1/2)	0.949	0.532

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	SMALL TUBES		293	cell-free crystallization