9UWS

Crystal structure of human galectin-10 produced by cell-free protein synthesis in complex with trehalose

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL32XU
Synchrotron site	SPring-8
Beamline	BL32XU
Temperature [K]	100
Detector technology	PIXEL
Collection date	2023-02-01
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	1
Spacegroup name	P 65 2 2
Unit cell lengths	49.150, 49.150, 262.180
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	28.120 - 1.780
R-factor	0.2157
Rwork	0.212
R-free	0.24800
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.009
RMSD bond angle	1.151
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	MOLREP
Refinement software	PHENIX (1.19.2_4158)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	1.890
High resolution limit [Å]	1.780	1.780
Number of reflections	18544	3009
<I/σ(I)>	11
Completeness [%]	96.0	97.6
Redundancy	5.8
CC(1/2)	0.992	0.543

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	SMALL TUBES		293	cell-free crystallization