9UWK

Crystal structure of human galectin-10 produced by cell-free protein synthesis

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL32XU
Synchrotron site	SPring-8
Beamline	BL32XU
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-07-28
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	1
Spacegroup name	P 65 2 2
Unit cell lengths	49.280, 49.280, 261.890
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	35.750 - 1.600
R-factor	0.2002
Rwork	0.199
R-free	0.22050
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.008
RMSD bond angle	0.997
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	MOLREP
Refinement software	PHENIX (1.19.2_4158)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	1.660
High resolution limit [Å]	1.600	1.600
Number of reflections	26219	2531
<I/σ(I)>	18.5
Completeness [%]	100.0	100
Redundancy	54
CC(1/2)	1.000	0.775

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	SMALL TUBES		293	cell-free crystallization