9UVG

Crystal structure of Sec23a/24a/22b bound to mouse STING pSGMD motif

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL19U1
Synchrotron site	SSRF
Beamline	BL19U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2023-04-14
Detector	DECTRIS EIGER R 4M
Wavelength(s)	0.987
Spacegroup name	C 1 2 1
Unit cell lengths	148.003, 96.912, 129.602
Unit cell angles	90.00, 90.07, 90.00

Refinement procedure

Resolution	21.430 - 2.540
R-factor	0.2259
Rwork	0.225
R-free	0.25320
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.003
RMSD bond angle	0.574
Data reduction software	HKL-3000
Data scaling software	HKL-3000
Phasing software	PHASER
Refinement software	PHENIX ((1.21.2_5419: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	21.430	2.590
High resolution limit [Å]	2.540	2.540
Number of reflections	53285	1308
<I/σ(I)>	3.8
Completeness [%]	86.5
Redundancy	3.6
CC(1/2)	0.860	0.660

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		298	50 mM Tris, pH 7.8, 0.3 M NaAc, 20% PEG4000