9UVF
Crystal structure of Sec23a/24a/22b bound to mouse STING LI motif
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL19U1 |
| Synchrotron site | SSRF |
| Beamline | BL19U1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2023-05-01 |
| Detector | DECTRIS EIGER R 4M |
| Wavelength(s) | 0.987 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 148.291, 95.500, 130.778 |
| Unit cell angles | 90.00, 90.12, 90.00 |
Refinement procedure
| Resolution | 48.990 - 3.150 |
| R-factor | 0.268 |
| Rwork | 0.265 |
| R-free | 0.32000 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.002 |
| RMSD bond angle | 0.583 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.21.2_5419: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 48.990 | 3.230 |
| High resolution limit [Å] | 3.150 | 3.150 |
| Number of reflections | 31307 | 2012 |
| <I/σ(I)> | 4.6 | |
| Completeness [%] | 98.6 | |
| Redundancy | 8.6 | |
| CC(1/2) | 0.820 | 0.690 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 298 | 50 mM Tris, pH 7.8, 0.3 M NaAc, 20% PEG4000 |
