9UVE
Crystal structure of Sec23a/24a/22b bound to STING FpS motif
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL19U1 |
| Synchrotron site | SSRF |
| Beamline | BL19U1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2023-05-01 |
| Detector | DECTRIS EIGER R 4M |
| Wavelength(s) | 0.987 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 149.264, 98.170, 130.122 |
| Unit cell angles | 90.00, 90.37, 90.00 |
Refinement procedure
| Resolution | 19.880 - 2.600 |
| R-factor | 0.2343 |
| Rwork | 0.233 |
| R-free | 0.29150 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.711 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.21.2_5419: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 19.880 | 2.640 |
| High resolution limit [Å] | 2.600 | 2.600 |
| Number of reflections | 111527 | 4698 |
| <I/σ(I)> | 5.6 | |
| Completeness [%] | 98.4 | |
| Redundancy | 8.6 | |
| CC(1/2) | 0.900 | 0.170 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 298 | 50 mM Tris, pH 7.8, 0.3 M NaAc, 10% PEG4000 |
