9UUL

Crystal structure of the mu2 subunit of the clathrin-adaptor protein 2 (AP2) bound to HPV16 E7(residues 22-39; S31E and S32E)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PAL/PLS BEAMLINE 5C (4A)
Synchrotron site	PAL/PLS
Beamline	5C (4A)
Temperature [K]	93
Detector technology	PIXEL
Collection date	2024-10-23
Detector	DECTRIS EIGER X 9M
Wavelength(s)	0.987
Spacegroup name	P 64
Unit cell lengths	125.569, 125.569, 74.251
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	43.869 - 3.298
R-factor	0.1967
Rwork	0.194
R-free	0.22470
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.011
RMSD bond angle	1.131
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	MOLREP
Refinement software	PHENIX ((1.10.1_2155: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	3.360
High resolution limit [Å]	3.298	3.298
Rmerge	0.058	0.300
Number of reflections	9969	457
<I/σ(I)>	15.7
Completeness [%]	97.8
Redundancy	5.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		291	3.5 M ammonium chloride and 100 mM sodium acetate trihydrate (pH 4.5)