9UUJ

Crystal structure of the mu2 subunit of the clathrin-adaptor protein 2 (AP2) bound to HPV16 E7(residues 22-39)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PAL/PLS BEAMLINE 5C (4A)
Synchrotron site	PAL/PLS
Beamline	5C (4A)
Temperature [K]	93
Detector technology	PIXEL
Collection date	2024-10-23
Detector	DECTRIS EIGER X 9M
Wavelength(s)	0.987
Spacegroup name	P 64
Unit cell lengths	125.094, 125.094, 73.588
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	40.947 - 3.703
R-factor	0.2098
Rwork	0.207
R-free	0.23360
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.003
RMSD bond angle	0.618
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	MOLREP
Refinement software	PHENIX ((1.10.1_2155: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	3.760
High resolution limit [Å]	3.700	3.700
Rmerge	0.074	0.477
Number of reflections	6757	340
<I/σ(I)>	21
Completeness [%]	94.7
Redundancy	7.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		291	2.0 M sodium formate and 100 mM sodium acetate trihydrate (pH 4.6)