9UU2
Outer membrane protein OmpA-c-like domain
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL18U1 |
Synchrotron site | SSRF |
Beamline | BL18U1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2024-01-27 |
Detector | DECTRIS PILATUS3 6M |
Wavelength(s) | 0.987 |
Spacegroup name | P 31 2 1 |
Unit cell lengths | 44.369, 44.369, 102.746 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 22.180 - 1.900 |
R-factor | 0.2167 |
Rwork | 0.213 |
R-free | 0.24830 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.005 |
RMSD bond angle | 0.734 |
Data reduction software | HKL-3000 |
Data scaling software | HKL-3000 (7.21) |
Phasing software | PHENIX |
Refinement software | PHENIX (1.17.1_3660) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 22.810 | 1.968 |
High resolution limit [Å] | 1.900 | 1.900 |
Rmeas | 0.127 | 1.248 |
Number of reflections | 9756 | 9756 |
<I/σ(I)> | 1.16 | 1.16 |
Completeness [%] | 99.6 | |
Redundancy | 16.6 | |
CC(1/2) | 0.762 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 300 | 1% Tryptone, 0.001M sodium azide, 0.05M HEPES sodium pH 7.0, 20% w/v PEG3350. |