9UU1

Bacteroides fragilis T6SS immunity protein BtiO

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL18U1
Synchrotron site	SSRF
Beamline	BL18U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2022-03-27
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.987
Spacegroup name	P 32 2 1
Unit cell lengths	41.251, 41.251, 124.961
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	23.520 - 2.000
R-factor	0.2074
Rwork	0.204
R-free	0.24130
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.008
RMSD bond angle	0.787
Data reduction software	HKL-3000
Data scaling software	HKL-3000 (7.21)
Phasing software	PHENIX
Refinement software	PHENIX (1.17.1_3660)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	23.520	2.072
High resolution limit [Å]	2.000	2.000
Rmeas		0.683
Number of reflections	8809	8809
<I/σ(I)>	1.5
Completeness [%]	96.7
Redundancy	14.3
CC(1/2)	0.993

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		300	20% v/v 2-propanol, 0.1M MES monohydrate pH 6.0, 20% w/v PEG MME 2000.