9URH

Structural insight into DNA recognition by RRM1+2 domain of human ETR-3 protein

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID23-1
Synchrotron site	ESRF
Beamline	ID23-1
Temperature [K]	80
Detector technology	PIXEL
Collection date	2022-01-21
Detector	DECTRIS PILATUS 6M-F
Wavelength(s)	0.8856
Spacegroup name	I 2 2 2
Unit cell lengths	45.116, 70.770, 134.009
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	37.770 - 1.820
R-factor	0.1981
Rwork	0.197
R-free	0.22130
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4lmz
RMSD bond length	0.007
RMSD bond angle	0.838
Data reduction software	autoPROC
Data scaling software	autoPROC
Phasing software	PHENIX
Refinement software	PHENIX (1.21.1_5286)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	37.774	1.854
High resolution limit [Å]	1.820	1.823
Rmerge	0.090	1.172
Rpim		0.329
Number of reflections	19591	965
<I/σ(I)>	15.3
Completeness [%]	99.9	100
Redundancy	12.8	13.5
CC(1/2)	0.997	0.787

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		277	0.2 M Magnesium acetate trihydrate, 0.1 M Sodium cacodylate trihydrate pH 6.5, 20% w/v Polyethylene glycol 8,000