9UP8

Tacheng tick virus 1 nucleoprotein

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL18U1
Synchrotron site	SSRF
Beamline	BL18U1
Temperature [K]	289
Detector technology	CCD
Collection date	2021-05-12
Detector	ADSC QUANTUM 315
Wavelength(s)	0.979
Spacegroup name	C 1 2 1
Unit cell lengths	154.557, 78.747, 92.636
Unit cell angles	90.00, 107.60, 90.00

Refinement procedure

Resolution	50.000 - 2.520
R-factor	0.2256
Rwork	0.223
R-free	0.27250
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.010
RMSD bond angle	1.151
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX (1.19.2_4158)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.670
High resolution limit [Å]	2.520	2.520
Number of reflections	35656	35627
<I/σ(I)>	12.4
Completeness [%]	98.0
Redundancy	3.4
CC(1/2)	0.993	0.873

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		289	0.1 M Lithium sulfate monohydrate, 0.1 M Sodium citrate tribasic dihydrate pH 5.5, 20% w/v Polyethylene glycol 1,000