9UP5

Crystal structure of LapB from Klebsiella pneumoniae

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PAL/PLS BEAMLINE 5C (4A)
Synchrotron site	PAL/PLS
Beamline	5C (4A)
Temperature [K]	125
Detector technology	PIXEL
Collection date	2021-06-02
Detector	DECTRIS EIGER X 9M
Wavelength(s)	0.97959
Spacegroup name	C 1 2 1
Unit cell lengths	134.780, 109.070, 81.930
Unit cell angles	90.00, 125.48, 90.00

Refinement procedure

Resolution	28.750 - 3.440
R-factor	0.2308
Rwork	0.229
R-free	0.26890
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.003
RMSD bond angle	0.616
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHENIX
Refinement software	PHENIX (1.19.1_4122)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	28.750	3.563
High resolution limit [Å]	3.440	3.440
Rmerge	0.087
Number of reflections	12881	1291
<I/σ(I)>	11.51
Completeness [%]	99.6
Redundancy	4.6
CC(1/2)		0.082

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293.15	10% (w/v) PEG 8000, 0.1M Na/K phosphate pH6.2, 0.2M NaCl