9UP4

Crystal structure of Ebola virus VP30 and its peptide RPL1

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL10U2
Synchrotron site	SSRF
Beamline	BL10U2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2024-05-26
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.97915
Spacegroup name	P 65
Unit cell lengths	111.973, 111.973, 48.932
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	55.990 - 2.760
R-factor	0.2621
Rwork	0.260
R-free	0.31280
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.003
RMSD bond angle	0.616
Data reduction software	DIALS
Data scaling software	DIALS
Phasing software	PHASER
Refinement software	PHENIX (1.21.2_5419)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	55.990	3.160
High resolution limit [Å]	2.760	2.760
Number of reflections	9210	2143
<I/σ(I)>	11.1
Completeness [%]	98.1
Redundancy	1.9
CC(1/2)	0.984	0.516

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	0.1 M Sodium malonate pH5.0, 12% w/v polyethylene glycol 3350