9UOW
Crystal structure of Gossypium hirsutum (Cotton) 5-enol-pyruvyl-shikimate-3-phosphate synthase (EPSPS) in open conformation
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 HF |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2024-08-17 |
| Detector | RIGAKU HyPix-6000HE |
| Wavelength(s) | 1.54184 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 79.855, 89.942, 116.233 |
| Unit cell angles | 90.00, 107.32, 90.00 |
Refinement procedure
| Resolution | 24.470 - 2.200 |
| R-factor | 0.20349 |
| Rwork | 0.201 |
| R-free | 0.24769 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 7pxy |
| RMSD bond length | 0.016 |
| RMSD bond angle | 2.435 |
| Data reduction software | CrysalisPro |
| Data scaling software | Aimless |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0425) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 24.470 | 2.240 |
| High resolution limit [Å] | 2.200 | 2.200 |
| Rmerge | 0.070 | |
| Rmeas | 0.082 | |
| Number of reflections | 79755 | 4526 |
| <I/σ(I)> | 14.1 | 2.2 |
| Completeness [%] | 99.9 | |
| Redundancy | 6.5 | |
| CC(1/2) | 0.998 | 0.782 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.4 | 293 | 0.16 M calcium acetate hydrate, 0.08 M sodium cacodylate (pH 6.5), and 14.4% w/v PEG 8000 |
