9UON

Tacheng tick virus 1 nucleoprotein head domain with selenomethionine

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL18U1
Synchrotron site	SSRF
Beamline	BL18U1
Temperature [K]	289
Detector technology	PIXEL
Collection date	2021-04-12
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.978
Spacegroup name	P 65
Unit cell lengths	113.166, 113.166, 54.117
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	37.040 - 1.550
R-factor	0.17
Rwork	0.169
R-free	0.19870
Structure solution method	MAD
RMSD bond length	0.011
RMSD bond angle	1.142
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	AutoSol
Refinement software	PHENIX (1.19.2_4158)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	1.610
High resolution limit [Å]	1.550	1.550
Number of reflections	103756	57834
<I/σ(I)>	11.8
Completeness [%]	100.0
Redundancy	19.9
CC(1/2)	0.969	0.999

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		289.15	0.2M LiSO4,20%PEG 3350,