9UOL

Cryo-EM structure of pyrene-modified TIP60 double mutant (G12C/S50C) with addition of Nile Red

This is a non-PDB format compatible entry.

EM specimen

Vitrification	Yes
Instrument	FEI VITROBOT MARK IV

EM Data Collection

Microscope	FEI TALOS ARCTICA
Electron Gun	FIELD EMISSION GUN
Accelerating Voltage (kV)	200
Cs (mm)	2.7
Detector	FEI FALCON IV (4k x 4k)

3D Reconstruction

Method	SINGLE PARTICLE
Resolution (Å)	3.8
Number of Particle	235783
Software	Topaz,RELION,EPU,CTFFIND,Coot,PHENIX
Symmetry	I
CTF correction	PHASE FLIPPING AND AMPLITUDE CORRECTION