9UO0

Crystal structure of nanobody Tnb165 with MERS-CoV RBD

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL02U1
Synchrotron site	SSRF
Beamline	BL02U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2024-11-16
Detector	DECTRIS EIGER2 S 9M
Wavelength(s)	0.97918
Spacegroup name	P 61 2 2
Unit cell lengths	88.100, 88.100, 235.680
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	37.660 - 2.500
Rwork	0.226
R-free	0.25030
Structure solution method	MOLECULAR REPLACEMENT
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX (v.1.18.2)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	40.100	2.560
High resolution limit [Å]	2.500	2.500
Rmerge	0.053	3.159
Rmeas	0.376	3.235
Rpim	0.081	0.697
Number of reflections	19650	1393
<I/σ(I)>	16.6
Completeness [%]	100.0
Redundancy	38.4
CC(1/2)	0.999	0.684

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		291.15	0.1 M MES pH6.5, 15% v/v PEG 550 MME