9UMN
Giraffe KIF5A motor domain crystallized in the presence of 1 mM AMPPNP
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06DA |
| Synchrotron site | SLS |
| Beamline | X06DA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2017-09-01 |
| Detector | DECTRIS PILATUS 2M |
| Wavelength(s) | 1.0000 |
| Spacegroup name | P 1 |
| Unit cell lengths | 51.060, 51.060, 77.140 |
| Unit cell angles | 100.39, 108.06, 93.30 |
Refinement procedure
| Resolution | 45.640 - 1.850 |
| R-factor | 0.1993 |
| Rwork | 0.197 |
| R-free | 0.24000 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.845 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | PHENIX (v2.8) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.960 |
| High resolution limit [Å] | 1.850 | 1.850 |
| Rmeas | 0.083 | 0.565 |
| Number of reflections | 60002 | 34941 |
| <I/σ(I)> | 11.2 | |
| Completeness [%] | 96.9 | |
| Redundancy | 3.55 | |
| CC(1/2) | 0.997 | 0.861 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5 | 293 | 1.8-2.0 M ammonium sulfate 0.2-0.5 M di-ammonium tartrate 0.1 M sodium acetate (pH 5.0) |
