9UMM

Giraffe KIF5A motor domain crystallized in the presence of 10 mM ADP

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X06DA
Synchrotron site	SLS
Beamline	X06DA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-09-01
Detector	DECTRIS PILATUS 2M
Wavelength(s)	1.0000
Spacegroup name	P 1
Unit cell lengths	50.330, 50.310, 78.930
Unit cell angles	99.59, 107.25, 94.73

Refinement procedure

Resolution	47.390 - 1.950
R-factor	0.229
Rwork	0.227
R-free	0.25970
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.007
RMSD bond angle	0.883
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	PHENIX (v2.8)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	47.390	2.070
High resolution limit [Å]	1.950	1.950
Number of reflections	51367	8186
<I/σ(I)>	9.69
Completeness [%]	97.4
Redundancy	3.54
CC(1/2)	0.998	1.000

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	1.8 M ammonium sulfate 0.4-0.5 M di-ammonium tartrate 0.1 M sodium acetate pH 5.0