9UMC

Human poly ADP-ribose polymerase(PARP-1 )zinc finger 1 bound to 13bp DNA

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL19U1
Synchrotron site	SSRF
Beamline	BL19U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2024-06-06
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.97955
Spacegroup name	P 64 2 2
Unit cell lengths	86.960, 86.960, 150.150
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	43.520 - 2.600
R-factor	0.2058
Rwork	0.203
R-free	0.25757
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.010
RMSD bond angle	1.637
Data reduction software	autoPROC
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	REFMAC (5.8.0430)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	48.380	2.720
High resolution limit [Å]	2.600	2.600
Number of reflections	10947	1309
<I/σ(I)>	30.1
Completeness [%]	99.9
Redundancy	1.8
CC(1/2)	0.999	0.974

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		289	4M Sodium Formate