9ULM

Mogamulizumab in complex with CCR4 N-terminus peptide (N2-C29)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PAL/PLS BEAMLINE 5C (4A)
Synchrotron site	PAL/PLS
Beamline	5C (4A)
Temperature [K]	100
Detector technology	PIXEL
Collection date	2024-12-30
Detector	DECTRIS EIGER X 9M
Wavelength(s)	1
Spacegroup name	P 1
Unit cell lengths	65.390, 71.190, 73.450
Unit cell angles	81.24, 64.41, 71.05

Refinement procedure

Resolution	29.550 - 2.010
R-factor	0.1989
Rwork	0.197
R-free	0.24400
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.007
RMSD bond angle	0.885
Data reduction software	XDS
Data scaling software	XDS
Phasing software	MOLREP
Refinement software	PHENIX ((1.20_4459: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.070
High resolution limit [Å]	2.010	2.010
Number of reflections	70512	3523
<I/σ(I)>	7.3
Completeness [%]	93.5
Redundancy	1.8
CC(1/2)	0.995	0.792

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION		293	0.1 M Sodium HEPES pH 6.5, 20% (W/V) PEG 10000