9ULB
Cryogenic temperature crystal structure of Nmar_1308 protein at 3.29 angstrom resolution
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SPRING-8 BEAMLINE BL32XU |
Synchrotron site | SPring-8 |
Beamline | BL32XU |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2023-11-17 |
Detector | DECTRIS EIGER X 9M |
Wavelength(s) | 1 |
Spacegroup name | P 32 1 2 |
Unit cell lengths | 76.300, 76.300, 210.110 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 41.120 - 3.290 |
R-factor | 0.3621 |
Rwork | 0.356 |
R-free | 0.41470 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.004 |
RMSD bond angle | 0.750 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHENIX |
Refinement software | PHENIX ((1.19.2_4158: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 41.120 | 3.490 |
High resolution limit [Å] | 3.290 | 3.290 |
Number of reflections | 10985 | 1754 |
<I/σ(I)> | 6.06 | |
Completeness [%] | 99.9 | |
Redundancy | 13.19 | |
CC(1/2) | 0.980 | 0.450 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | BATCH MODE | 298 | 0.3M Sodium fluoride; 0.3M Sodium bromide, 0.3M Sodium iodide, 1.0M Tris (base), BICINE pH 8.5, 25% v/v MPD, 25% PEG 1000, 25% w/v PEG 3350 |