9UL9

Cryogenic temperature crystal structure of Nmar_1308 protein at 2.71 angstrom resolution

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL32XU
Synchrotron site	SPring-8
Beamline	BL32XU
Temperature [K]	100
Detector technology	PIXEL
Collection date	2023-11-17
Detector	DECTRIS EIGER X 9M
Wavelength(s)	1
Spacegroup name	P 32
Unit cell lengths	74.190, 74.190, 206.850
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	40.290 - 2.710
R-factor	0.3719
Rwork	0.370
R-free	0.39600
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.002
RMSD bond angle	0.476
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHENIX
Refinement software	PHENIX ((1.19.2_4158: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	40.290	2.870
High resolution limit [Å]	2.710	2.710
Number of reflections	34665	5506
<I/σ(I)>	8.19
Completeness [%]	100.0
Redundancy	3.8
CC(1/2)	0.960	0.620

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	BATCH MODE		298	0.3M Sodium nitrate, 0.3 Sodium phosphate dibasic, 0.3M Ammonium sulfate, 1.0 M Tris (base), BICINE pH 8.5, 25% v/v MPD, 25% PEG 1000, 25% w/v PEG 3350