9UL6

Crystal structure of Lamin A/C Ig-like domain mutant - R435C

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PAL/PLS BEAMLINE 5C (4A)
Synchrotron site	PAL/PLS
Beamline	5C (4A)
Temperature [K]	80
Detector technology	PIXEL
Collection date	2022-10-20
Detector	DECTRIS EIGER X 9M
Wavelength(s)	0.9795
Spacegroup name	P 64 2 2
Unit cell lengths	92.007, 92.007, 204.805
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	34.410 - 2.500
R-factor	0.2103
Rwork	0.203
R-free	0.27550
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	7crg
RMSD bond length	0.008
RMSD bond angle	0.946
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX (1.21rc1_4933)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.540
High resolution limit [Å]	2.500	2.500
Rmeas	0.093	0.359
Rpim	0.019	0.070
Number of reflections	17639	855
<I/σ(I)>	34.1
Completeness [%]	94.8
Redundancy	17.6
CC(1/2)	0.999	0.981

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		287	0.1 M Sodium citrate tribasic dihydrate pH 5.0, 30% v/v Jeffamine ED-2001 pH 7.0