9UKQ
Crystal structure of glycogen phosphorylase from Escherichia coli
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL44XU |
| Synchrotron site | SPring-8 |
| Beamline | BL44XU |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2024-11-27 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.9 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 134.769, 205.603, 264.075 |
| Unit cell angles | 90.00, 104.35, 90.00 |
Refinement procedure
| Resolution | 46.210 - 3.500 |
| R-factor | 0.2329 |
| Rwork | 0.231 |
| R-free | 0.27310 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.665 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | MOLREP |
| Refinement software | PHENIX ((1.19.2_4158: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 47.690 | 3.560 |
| High resolution limit [Å] | 3.500 | 3.500 |
| Rmerge | 0.132 | 0.949 |
| Rmeas | 0.157 | 1.112 |
| Rpim | 0.083 | 0.576 |
| Number of reflections | 86898 | 4462 |
| <I/σ(I)> | 6.2 | |
| Completeness [%] | 99.1 | 99.9 |
| Redundancy | 3.5 | 3.7 |
| CC(1/2) | 0.994 | 0.596 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6 | 293 | Protein conc: 10.4mg/mL; 0.1M MES pH 6.0, 9% (v/v) PEG 3350 |
