9UJY

The structure of Egalitarian in complex with the K10 mRNA localization signal reveals a modular binding surface required for function

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X10SA
Synchrotron site	SLS
Beamline	X10SA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-03-11
Detector	DECTRIS PILATUS 6M
Wavelength(s)	1
Spacegroup name	P 21 21 21
Unit cell lengths	53.001, 82.311, 120.561
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	48.630 - 2.260
R-factor	0.1933
Rwork	0.193
R-free	0.20590
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1anf
RMSD bond length	0.017
RMSD bond angle	2.028
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX (1.21.2_5419)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.340
High resolution limit [Å]	2.200	2.200
Rmeas		1.370
Number of reflections	50894	8163
<I/σ(I)>	0.12
Completeness [%]	99.2
Redundancy	3.08
CC(1/2)	0.996	0.354

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	EVAPORATION		277	100 mM Tris-HCl, pH 7.5, 18% (w/v) PEG 6000, 50 mM MgCl2