9UJU
The structure of Egalitarian in complex with the K10 mRNA localization signal reveals a modular binding surface required for function
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X10SA |
| Synchrotron site | SLS |
| Beamline | X10SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2020-04-13 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 1 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 68.504, 106.799, 139.817 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 44.480 - 3.080 |
| R-factor | 0.2425 |
| Rwork | 0.240 |
| R-free | 0.29160 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | ITasser |
| RMSD bond length | 0.014 |
| RMSD bond angle | 2.026 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.17.1_3660: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 3.270 |
| High resolution limit [Å] | 3.080 | 3.080 |
| Rmeas | 1.410 | |
| Number of reflections | 36673 | 5931 |
| <I/σ(I)> | 0.17 | |
| Completeness [%] | 99.8 | |
| Redundancy | 7.02 | |
| CC(1/2) | 0.999 | 0.702 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | EVAPORATION | 293 | 50 mM sodium citrate, pH 4.8, 30% (w/v) MPD, 10 mM CaCl2, 2.5 mM Spermine |
