9UJJ
Crystal structure of the BTB domain mouse Keap1 in complex with CDDO-Me
This is a non-PDB format compatible entry.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL44XU |
| Synchrotron site | SPring-8 |
| Beamline | BL44XU |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2023-12-05 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.9 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 324.477, 72.101, 92.679 |
| Unit cell angles | 90.00, 103.29, 90.00 |
Refinement procedure
| Resolution | 46.260 - 3.430 |
| R-factor | 0.2554 |
| Rwork | 0.252 |
| R-free | 0.32000 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.070 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.21_5207) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 46.260 | 3.553 |
| High resolution limit [Å] | 3.430 | 3.430 |
| Number of reflections | 27690 | 2672 |
| <I/σ(I)> | 16.47 | |
| Completeness [%] | 96.3 | |
| Redundancy | 3.4 | |
| CC(1/2) | 1.000 | 0.956 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 277 | 100mM AAB (pH 7.0), 6% PEG 20000, 200mM Ammonium sulfate, 100mM arginine, 100mM Glutamic acid |
