9UJG

Crystal structure of the BTB domain mouse Keap1

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL44XU
Synchrotron site	SPring-8
Beamline	BL44XU
Temperature [K]	100
Detector technology	PIXEL
Collection date	2022-07-28
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.9
Spacegroup name	P 65 2 2
Unit cell lengths	42.819, 42.819, 261.896
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	37.080 - 2.800
R-factor	0.2243
Rwork	0.222
R-free	0.26020
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.010
RMSD bond angle	1.108
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	PHENIX (1.21_5207)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	37.080	2.960
High resolution limit [Å]	2.800	2.800
Number of reflections	4087	1069
<I/σ(I)>	16.11
Completeness [%]	99.7
Redundancy	7.6
CC(1/2)	0.999	0.724

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		277	100mM Bis-Tris Propane (pH 6.5), 200mM LiCl, 13% PEG 3350, 4% Sucrose