9UJ3

Improved thermostability of a GH10 xylanase based on its X-ray crystal structure

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17B1
Synchrotron site	SSRF
Beamline	BL17B1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-05-19
Detector	DECTRIS PILATUS3 S 2M
Wavelength(s)	0.97854
Spacegroup name	P 1 21 1
Unit cell lengths	53.077, 68.492, 90.538
Unit cell angles	90.00, 92.67, 90.00

Refinement procedure

Resolution	33.660 - 1.910
R-factor	0.16058
Rwork	0.159
R-free	0.19305
Structure solution method	MOLECULAR REPLACEMENT
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	REFMAC (5.8.0419)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	1.940
High resolution limit [Å]	1.910	1.910
Number of reflections	49826	49826
<I/σ(I)>	18.722
Completeness [%]	99.2
Redundancy	6.5
CC(1/2)	0.993	0.919

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION		297	0.16MCH3COONa,0.1MTris-HCl pH 8.5,28% PEG4000