9UJ2
14-3-3 zeta chimera with the S202R peptide of SARS-CoV-2 N (residues 200-213)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2024-04-15 |
| Detector | RIGAKU HyPix-6000HE |
| Wavelength(s) | 1.54184 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 60.555, 84.452, 139.873 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 21.760 - 1.800 |
| R-factor | 0.19667 |
| Rwork | 0.195 |
| R-free | 0.23302 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.012 |
| RMSD bond angle | 2.079 |
| Data reduction software | CrysalisPro |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0430) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 21.760 | 1.840 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Rmerge | 0.106 | 0.857 |
| Rmeas | 0.111 | 0.935 |
| Rpim | 0.035 | 0.367 |
| Total number of observations | 676288 | 23631 |
| Number of reflections | 67049 | 3849 |
| <I/σ(I)> | 15 | 2.1 |
| Completeness [%] | 99.7 | |
| Redundancy | 10.1 | 6.1 |
| CC(1/2) | 0.999 | 0.725 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 288 | 1.8M Ammonium sulfate, 0.1M BIS-TRIS pH 6.5, 2% v/v Polyethylene glycol monomethyl ether 550 |
